Intrinsically disordered regions affect the expression of Toll-like receptor TIR domains in soluble form
- Авторлар: Lushpa V.A.1, Goncharuk М.V.1, Arseniev A.S.1, Mineev К.S.1,2, Goncharuk S.A.1
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Мекемелер:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt
- Шығарылым: Том 51, № 3 (2025)
- Беттер: 523-530
- Бөлім: ПИСЬМА РЕДАКТОРУ
- URL: https://rjonco.com/0132-3423/article/view/687051
- DOI: https://doi.org/10.31857/S0132342325030145
- EDN: https://elibrary.ru/KRUWDK
- ID: 687051
Дәйексөз келтіру
Аннотация
Here we investigate the effect of the C-terminal region on the solubility of TLR4 and TLR10 TIR domains upon the heterologous expression in Escherichia coli. We demonstrated that the absence of this region significantly enhances the yields of globular domains in a soluble form. These findings complement the protocols, previously established to synthesize the TLR TIR domains for the structural studies by various techniques, including by NMR spectroscopy.
Негізгі сөздер
Толық мәтін

Авторлар туралы
V. Lushpa
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 142290
М. Goncharuk
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 142290
A. Arseniev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 142290
К. Mineev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt
Email: ms.goncharuk@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 142290; Max-von-Laue-Strasse 9, Frankfurt am Main, 60438 Germany
S. Goncharuk
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: ms.goncharuk@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 142290
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