Russian Journal of OncologyRussian Journal of Oncology1028-99842412-9119Eco-Vector3996610.17816/onco39966Research ArticlePROGNOSTIC SIGNIFICANCE OF COMPUTER-AIDED PLOIDOMETRY IN RENAL CELL CARCINOMACherdantsevaT. Mdrakon@agmu.ruBobrovI. P-KlimachevV. V-BryukhanovV. M-LazarevA. F-AvdalyanA. M-DolgatovA. Yu.-KazartsevA. V-The Altay state of medical universityAltay branch of N.N.Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences15022013181323522072020Copyright © 2013, Eco-Vector2013We have investigated the prognostic significance of DNA computer morphometry in the nuclei of tumor cells in renal cell carcinoma (RCC). Surgical specimens of 108 patients with renal cell carcinoma was the material for investigation. The mean age was 57,8 ± 0,9 years. Males were 49 (45.3%), women - 59 (54.6%). Correlation relationship was revealed between index of DNA accumulation (INDNK) in the nuclei of tumor cells with clinical stage (r = 0,64; p = 0,0001), the size of the tumor node (r = 0,44; p = 0,0001), the presence of regional and distant metastases (r = 0,68; p = 0,0001), Fuhrman tumor grading (r = 0,75; p = 0,001). No relationship of INDNK with sex (r = 0,10; p = 0,30), age (r = 0,08; p = 0,41) and patients with histological types of tumors (r = 0,09; p = 0, 32) was revealed. During Cox multivariate regression analysis, the following independent predictors of RCC were revealed: the presence of regional and distant metastases (p = 0,0008), the degree of Fuhrman nucleus atypia (p = 0,005); stage by TNM (p = 0,03) and ploidy (p = 0.03). Such clinical - anatomical factors such as gender, age, tumor size and histological types of tumor didn;t have prognostic significance in assessing the 5 - year survival rate of patients. Thus, the ploidy of tumor cells is a significant prognostic parameter along with the classical prognostic factors in RCC.kidney cancerploidyforecastрак почкиплоидностьпрогноз[Budía Alba A., Gómez Pérez L., Bango V., Ruiz-Cerdá J. I., Sempere A., Queipo J. A., Jiménez Cruz J. F. Prognostic factors for disease progression in patients with renal cell carcinoma. Actas Urol. Esp. 2007; 31: 831—44.][Liu L., Zhang C. DNA ploidy analysis and Ki-67 examination on renal neoplasms: investigation of degree of malignancy and biological behavior of small renal tumors. Zhonghua Bing Li Xue Za Zhi. 2001; 30: 39—42.][Pinto A. E., Monteiro P., Silva G., Ayres J. V., Soares J. Prognostic biomarkers in renal cell carcinoma: relevance of DNA ploidy in predicting disease-related survival. Int. J. Biol. Markers. 2005; 20: 249—56.][Schafhauser W., Liedl T., Elsässer D., Zörcher T., Schrott K. M. Flow cytometric analysis of DNA-aneuploidy subgroups and proliferation in renal cell carcinoma. Anticancer Res. 1999; 19: 1471—5.][Eskelinen M., Lipponen P., Nordling S. Prognostic evaluation of DNA flow cytometry and histo-morphological criteria in renal cell carcinoma. Anticancer Res. 1995; 15: 2279—83.][Papadopoulos I., Rudolph P., Weichert-Jacobsen K. Value of p53 expression, cellular proliferation, and DNA content as prognostic indicators in renal cell carcinoma. Eur. Urol. 1997; 32: 110—7.][Skolarikos A., Alivizatos G., Bamias A., Mitropoulos D., Ferakis N., Deliveliotis C., Dimopoulos M. A. Bcl-2 protein and DNA ploidy in renal cell carcinoma: do they affect patient prognosis? Int. J. Urol. 2005; 12: 563—9.][Di Capua Sacoto C., Budia Alba A., Alapont Alacreu J. M., Ruiz Cerda J. L., Jimenez Cruz J. F. In vivo aneuploidization during the expansion of renal adenocarcinoma. Urol Int. 2011; 86: 466—9.][Feil G., Mittermuller B., Bichler K. H., Wunderer A., Wechsel H. W., Nelde H. J., St Krause F. DNA cytophotometry in renal cell carcinoma: a significant prognostic factor? Anticancer Res. 1999; 19: 1483—6.][Akhtar M., Chantziantoniou N. Quantitative image cell analysis of cytologic smears for DNA ploidy in renal parenchymal neoplasms. 1999; 21: 223—9.][Li G., Cottier M., Sabido O., Gentil-Perret A., Lambert C., Genin C., Tostain J. Different DNA ploidy patterns for the differentiation of common subtypes of renal tumors. Cell. Oncol. 2005; 27: 51—6.][Nenning H., Rassler J., Minh D. H. DNA cytometry in renal cell carcinoma. 1996; 141: 243—7.][Di Silverio F., Casale P., Colella D., Andrea L., Seccareccia F., Sciarra A. Independent value of tumor size and DNA ploidy for the prediction of disease progression in patients with organ-confined renal cell carcinoma. Cancer. 2000; 88: 835—43.][Li G., Cottier M., Sabido O., Gentil-Perret A., Lambert C., Passebosc-Faure K., Genin C., Tostain J. The in vivo DNA an-euploidization during expansion of conventional renal cell carcinoma. In Vivo. 2002; 16: 341—4.][Abou-Rebyeh H., Borgmann V., Nagel R., Al-Abadi H. DNA ploidy is a valuable predictor for prognosis of patients with resected renal cell carcinoma. Cancer. 2001; 92: 2280—85.][Minervini A., Di Cristofano C., Collecchi P., Salinitri G., Selli C., Bevilacqua G., Minervini R., Cavazzana A. Intracapsular clear cell renal carcinoma: ploidy status improves the prognostic value of the 2002 TNM classification. J. Urol. 2005; 174: 1203—7.][Nativ O., Sabo E., Madeb R., Halachmi S., Madjar S., Moskovitz B. Prognostic score for patients with localized renal cell carcinoma treated by nephrectomy. Isr. Med. Assoc. J. 2001; 3: 24—7.][Tannapfel A., Hahn H. A., Katalinic A., Fietkau R. J., Kgjhn R., Wittekind C. W. Prognostic value of ploidy and proliferation markers in renal cell carcinoma. Cancer. 1996; 77: 164—71.]