Characteristics of the Fatty Acid Composition of the Vacuolar Membrane Lipids Under the Conditions of Stress Induced by Copper Ions

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Abstract

The effect of different concentrations of copper ions (100 and 500 µM) on the fatty acid composition of the vacuolar membrane lipids of beet root tissues (Beta vulgaris L.) was studied. Exposure to 100 μM copper led to an increase in the content of FAs by 57 μg/mg of total lipids as compared to the control. Stearoyl desaturase (SDR) activity decreased from 0.87 in the control to 0.77 at 100 μM copper. Exposure to 500 μM copper caused an increase in the SDR activity up to 0.93, but the proportion of FAs decreased by 50 μg/mg of total lipids compared with 100 μM copper. In addition, there was an increase in the saturation of tonoplast lipids to 44 and 40% at 100 and 500 μM Cu²⁺, respectively. The results suggest that FAs of tonoplast lipids are involved in the stress response mechanisms induced by excessive Cu²⁺ concentrations.

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About the authors

I. S. Kapustina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
Russian Federation, Irkutsk, 664033

V. V. Gurina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: nichka.g@bk.ru
Russian Federation, Irkutsk, 664033

N. V. Ozolina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
Russian Federation, Irkutsk, 664033

E. V. Spiridonova

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
Russian Federation, Irkutsk, 664033

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