Use of a Fluorescent Dye for Controlling the Laser Absorption in the Femtosecond Laser Nanosurgery of Cells

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Abstract

The use of specific fluorescent dyes is able to reduce the labeled cell structure ionization threshold under the femtosecond laser impact. This feature may be applied in terms of the laser nanosurgery of the cell. In this work we use BioTracker Blue dye as a photosensitizer in order to receive an accurate control of cytoplasmic membrane ablation by femtosecond laser and to relief the laser-induced cell fusion. We have found that BioTracker Blue (366/441) increases an efficiency of the 760 and 730 nm laser absorption. However, an increase of absorption efficiency, provided by the BioTracker Blue staining, did not improved the efficiency of the cell fusion in the model systems: pairs of suspended A549 cells, oocytes and their polar bodies, and two-cell embryos.

About the authors

D. Yu Martirosyan

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

A. A Osychenko

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

A. D Zalesskiy

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

O. T Kalinina

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

U. A Tochilo

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

Yu. A Fedotov

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia; State Research Center–Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency, 123098, Moscow, Russia

Email: alina.chemphys@gmail.com

M. S Syrchina

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Email: alina.chemphys@gmail.com

V. A Nadtochenko

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

Author for correspondence.
Email: alina.chemphys@gmail.com

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