Bioavailable Nanocomposition of Chitosan-copper Nanoparticles as an Alternative to Antibiotics in BroilerFeed

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Pathogens pose a serious threat to agriculture as they reduce the growth rate and efficiency of farm birds, animals and cause economic losses. Therefore, there is a need for their use despite all the negative effects of antibiotics and bacterial resistance to them, and therefore, there is a need for effective alternatives that exclude the use of vaccines and drugs. An aggregatively stable nanocomposition of chitosan-nanoparticles of copper with an average size of the latter 25–30 nm was developed. The bactericidal effect of the nanocomposition was shown in vitro on pathogenic bacteria Enterococcus faecalis and investigated in vivo in the composition of broiler chickens’ drink and feed in comparison with antibiotic “Maxus”, used in their diet, on a wide range of pathogenic microorganisms. It was shown that the number of bacteria in broilers was 1.88% when the nanocomposition was administered, which is more than two times less compared to the group where the antibiotic was used.

Толық мәтін

Рұқсат жабық

Авторлар туралы

K. Apryatina

National Research Lobachevsky State University of Nizhni Novgorod

Хат алмасуға жауапты Автор.
Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603022

I. Egorov

Federal State Budget Scientific Institution Federal Scientific Center “All-Russian Research and Technological Poultry Institute”

Email: apryatina_kv@mail.ru
Ресей, Sergiev Posad, 141311

S. Zaitsev

National Research Lobachevsky State University of Nizhni Novgorod

Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603022

G. Laptev

BIOTROF LLC

Email: apryatina_kv@mail.ru
Ресей, Pushkin, St. Petersburg, 196602

E. Salomatina

National Research Lobachevsky State University of Nizhni Novgorod

Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603022

L. Smirnova

National Research Lobachevsky State University of Nizhni Novgorod

Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603022

A. Samodelkin

Scientific and educational center “Nizhny Novgorod REC”

Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603000

V. Frolov

AGROСHITIN LLC

Email: apryatina_kv@mail.ru
Ресей, Nizhny Novgorod, 603155

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2. Fig. 1. Plasmon absorption band of chitosan-stabilized copper NPS with MM2 × 105, [Cu] = 1 wt%.

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3. Fig. 2. Scheme of stabilization of copper NPS by chitosan functional groups.

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4. Fig. 3. Diffractogram of the chitosan-NP copper composition obtained in microwave radiation.

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5. Fig. 4. Absorption spectra of copper nanodispersions stabilized with chitosan with different MM: 1 — 0.2 × 105, 2 — 1×105, 3 — 2×105, [ Cu] = 1% by weight.

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6. 5. Diffractogram of the chitosan-NP copper composition, MM chitosan 1 × 105 (a), MM chitosan 2 × 105 (b).

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7. Fig. 6. The content of cellulolytics, %.

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8. 7. The content of bacilli (a), %; lactobacilli (b), %; bifidobacteria (c), %; PLA-synthesizing bacteria (d), %.м

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9. 8. Relative abundance of pathogens in the studied samples, %.

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