Email this article EFFECT OF ARYL HYDROCARBON RECEPTOR AGONISTS AND LIPOPOLYSACCHARIDE ON BENZO(A)PYRENE GENOTOXICITY MARKERS
- Authors: Babakov V.N.1, Rogovskaya N.Y.1, Kurdyukov I.D.1, Beltyukov P.P.1, Dulov S.A.1, Radilov A.S.1
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Affiliations:
- Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
- Issue: No 3 (2019)
- Pages: 19-25
- Section: Articles
- Published: 28.06.2019
- URL: https://rjonco.com/0869-7922/article/view/641186
- DOI: https://doi.org/10.36946/0869-7922-2019-3-19-25
- ID: 641186
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Abstract
The effect of aryl hydrocarbon receptor agonists (FICZ and ITE), as well as lipopolysaccharide under the toxic action of benzo(a)pyrene in HepaRG human hepatoma cells was evaluated. Active forms of the key stress-activated kinase cascades and DNA repair system proteins were used as markers of the genotoxic action of benzo(a)pyrene. A mixture of lipopolysaccharide with benzo(a)pyrene increases benzo(a)pyrene cytotoxicity and reduces the activation of DNA repair system proteins below the control level. Aryl hydrocarbon receptor agonists (FICZ and ITE) exhibit a cytoprotective effect against benzo(a) pyrene, enhance Akt1 kinase activation, and downregulate activation of the p53 protein and Chk1 and Chk2 checkpoint kinases. Thus, FICZ and ITE reduce the genotoxicity of benzo(a)pyrene.
About the authors
V. N. Babakov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Author for correspondence.
Email: babakov@rihophe.ru
Babakov Vladimir Nikolaevich
Saint Petersburg
N. Yu. Rogovskaya
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: fake@neicon.ru
Rogovskaya Nadezhda Yurievna
Saint Petersburg
I. D. Kurdyukov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: fake@neicon.ru
Kurdyukov Ivan Dmitrievich
Saint Petersburg
P. P. Beltyukov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: fake@neicon.ru
Beltyukov Petr Petrovich
Saint Petersburg
S. A. Dulov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: fake@neicon.ru
Dulov Sergei Anatolievich
Saint Petersburg
Russian FederationA. S. Radilov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: radilov@rihophe.ru
Radilov Andrei Stanislavovich
Saint Petersburg
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