Eco-friendly Biosynthesis of Ag-NPs by Streptomyces griseus with Anti-Candida albicans and Antitumor Activity


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Abstract

Background:The most significant sexually transmissible fungal disease, semen candidiasis, is caused by Candida albicans and impacts male reproductive potential. Actinomycetes are a group of microorganisms that could be isolated from various habitats and used for the biosynthesis of various nanoparticles with biomedical applications.

Objective:Testing antifungal activity of biosynthesized Ag nanoparticles versus isolated C. albicans from semen as well as its anticancer activity versus the Caco-2 cell line.

Methods:Screening 17 isolated actinomycetes for the biosynthesis of Ag nanoparticle biosynthesis. Characterization of biosynthesized nanoparticles, testing its anti-Candida albicans, and antitumor activity.

Results:Streptomyces griseus was the isolate that identified silver nanoparticles using UV, FTIR, XRD and TEM. Biosynthesized nanoparticles have promising anti-Candida albicans with MIC (125 ± 0.8) µg/ml and accelerate apoptotic rate versus Caco-2 cells (IC50 = 7.30 ± 0.54 µg/ml) with minimal toxicity (CC50 = 142.74 ± 4.71 µg/ml) versus Vero cells.

Conclusion:Certain actinomycetes could be used for the biosynthesis of nanoparticles with successive antifungal and anticancer activity to be verified by in vivo studies.

About the authors

Inas Abou El-Enain

Botany and Microbiology Department, Faculty of Science, Al Azhar University

Email: info@benthamscience.net

Nermine Abed

Botany and Microbiology Department, Faculty of Science, Al Azhar University

Email: info@benthamscience.net

Eman Helal

Department of Microbiology International Islamic Center for Population Studies and Research,, Al-Azhar University

Email: info@benthamscience.net

Eman Abdelkhalek

Botany and Microbiology Department, Faculty of Science, Al Azhar University

Email: info@benthamscience.net

Waleed Suleiman

Department of Microbiology, Faculty of Science,, Al Azhar University

Email: info@benthamscience.net

Nesreen Safwat

The Regional Center for Mycology and Biotechnology, Al-Azhar University

Email: info@benthamscience.net

Mohammed Yosri

The Regional Center for Mycology and Biotechnology,, Al Azhar University

Author for correspondence.
Email: info@benthamscience.net

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