Combined effects of cytokine TRAIL-based DR5-specific fusion protein with olaparib on tumor cell lines with different BRCA mutation status
- Авторлар: Yagolovich A.V.1, Isakova A.A.1,2, Kukovyakina E.V.2, Dolgikh D.A.1,2, Kirpichnikov M.P.1,2, Gasparian M.E.2
-
Мекемелер:
- Lomonosov Moscow State University
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Шығарылым: Том 51, № 3 (2025)
- Беттер: 398-407
- Бөлім: ОБЗОРНАЯ СТАТЬЯ
- URL: https://rjonco.com/0132-3423/article/view/686893
- DOI: https://doi.org/10.31857/S0132342325030034
- EDN: https://elibrary.ru/KQAJWF
- ID: 686893
Дәйексөз келтіру
Аннотация
Tumor cell death induction via activation of TRAIL (tumor necrosis factor-related apoptosis inducing ligand) cytokine signaling pathway is a promising strategy for anticancer therapy. Previously, we developed a fusion protein SRH-DR5-B-iRGD based on the DR5 (death receptor 5)-specific cytokine TRAIL variant DR5-B with antiangiogenic peptides. The SRH peptide specifically binds to the VEGFR2 (vascular endothelial growth factor receptor 2) receptor and blocks its VEGF-mediated activation; the iRGD peptide binds to integrin αvβ3 and the NRP-1 (neuropilin-1) receptor. All of these targets are known to be overexpressed on the surface of tumor cells. In the current study, we investigated the cytotoxic activity of the SRH-DR5-B-iRGD fusion protein in comparison with DR5-B in vitro in ovarian and breast adenocarcinoma cell lines with different BRCA mutation status in combination with a targeted poly(ADP-ribose) polymerase (PARP) inhibitor olaparib. Olaparib synergistically enhanced the cytotoxicity of TRAIL-based proteins regardless of the presence of BRCA mutations in the cells, and this effect was more pronounced for SRH-DR5-B-iRGD. Thus, the combination of SRH-DR5-B-iRGD with olaparib can be considered as a new approach to treatment of ovarian and breast adenocarcinomas regardless of the presence of BRCA mutations.
Толық мәтін

Авторлар туралы
A. Yagolovich
Lomonosov Moscow State University
Хат алмасуға жауапты Автор.
Email: yagolovichav@my.msu.ru
Faculty of Biology
Ресей, Leninskie Gory 1/12, Moscow, 119234A. Isakova
Lomonosov Moscow State University; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yagolovichav@my.msu.ru
Faculty of Biology
Ресей, Leninskie Gory 1/12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, Moscow, 117997E. Kukovyakina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yagolovichav@my.msu.ru
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 117997
D. Dolgikh
Lomonosov Moscow State University; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yagolovichav@my.msu.ru
Faculty of Biology
Ресей, Leninskie Gory 1/12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, Moscow, 117997M. Kirpichnikov
Lomonosov Moscow State University; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yagolovichav@my.msu.ru
Faculty of Biology
Ресей, Leninskie Gory 1/12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, Moscow, 117997M. Gasparian
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yagolovichav@my.msu.ru
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 117997
Әдебиет тізімі
- Sokolenko A.P., Sokolova T.N., Ni V.I., Preobrazhenskaya E.V., Iyevleva A.G., Aleksakhina S.N., Romanko A.A., Bessonov A.A., Gorodnova T.V., Anisimova E.I., Savonevich E.L., Bizin I.V., Stepanov I.A., Krivorotko P.V., Berlev I.V., Belyaev A.M., Togo A.V., Imyanitov E.N. // Breast Cancer Res. Treat. 2020. V. 184. P. 229–235. https://doi.org/10.1007/s10549-020-05827-8
- Arcieri M., Tius V., Andreetta C., Restaino S., Biasioli A., Poletto E., Damante G., Ercoli A., Driul L., Fagotti A., Lorusso D., Scambia G., Vizzielli G. // Front. Oncol. 2024. V. 14. P. 1335196. https://doi.org/10.3389/fonc.2024.1335196
- Jiang X., Li X., Li W., Bai H., Zhang Z. // J. Cell. Mol. Med. 2019. V. 23. P. 2303–2313. https://doi.org/10.1111/jcmm.14133
- Khaider N.G., Lane D., Matte I., Rancourt C., Piché A. // Am. J. Cancer Res. 2012. V. 2. P. 75–92.
- Mandal R., Raab M., Matthess Y., Becker S., Knecht R., Strebhardt K. // Molecular Oncology. 2014. V. 8. P. 232–249. https://doi.org/10.1016/j.molonc.2013.11.003
- Cuello M., Ettenberg S.A., Clark A.S., Keane M.M., Posner R.H., Nau M.M., Dennis P.A., Lipkowitz S. // Cancer Res. 2001. V. 61. P. 4892–4900.
- Kim D.-Y., Kim M.-J., Kim H.-B., Lee J.-W., Bae J.-H., Kim D.-W., Kang C.-D., Kim S.-H. // Biochim. Biophys. Acta. 2011. V. 1812. P. 796–805. https://doi.org/10.1016/j.bbadis.2011.04.004
- Jiang Q., Zhu H., Liang B., Huang Y., Li C. // Mol. Med. Rep. 2012. V. 6. P. 316–320. https://doi.org/10.3892/mmr.2012.902
- Gasparian M.E., Bychkov M.L., Yagolovich A.V., Kirpichnikov M.P., Dolgikh D.A. // Dokl. Biochem. Biophys. 2017. V. 477. P. 385–388. https://doi.org/10.1134/S1607672917060114
- Yin S., Xu L., Bandyopadhyay S., Sethi S., Reddy K.B. // Int. J. Oncol. 2011. V. 39. P. 891–898. https://doi.org/10.3892/ijo.2011.1085
- Zhou L., Wan Y., Zhang L., Meng H., Yuan L., Zhou S., Cheng W., Jiang Y. // Biomed. Pharmacother. 2024. V. 175. P. 116733. https://doi.org/0.1016/j.biopha.2024.116733
- Faraoni I., Aloisio F., De Gabrieli A., Consalvo M.I., Lavorgna S., Voso M.T., Lo-Coco F., Graziani G. // Cancer Lett. 2018. V. 423. P. 127–138. https://doi.org/10.1016/j.canlet.2018.03.008
- Ben Khaled N., Hammer K., Ye L., Alnatsha A., Widholz S.A., Piseddu I., Sirtl S., Schneider J., Munker S., Mahajan U.M., Montero J.J., Griger J., Mayerle J., Reiter F.P., De Toni E.N. // Cancers. 2022. V. 14. P. 5240. https://doi.org/10.3390/cancers14215240
- Bizzaro F., Fuso Nerini I., Taylor M.A., Anastasia A., Russo M., Damia G., Guffanti F., Guana F., Ostano P., Minoli L., Hattersley M., Arnold S., Ramos-Montoya A., Williamson S.C., Galbiati A., Urosevic J., Leo E., Cavallaro U., Ghilardi C., Barry S.T., Bani M.R., Giavazzi R. // J. Hematol. Oncol. 2021. V. 14. P. 186. https://doi.org/10.1186/s13045-021-01196-x
- Gasparian M.E., Chernyak B.V., Dolgikh D.A., Yagolovich A.V., Popova E.N., Sycheva A.M., Moshkovskii S.A., Kirpichnikov M.P. // Apoptosis. 2009. V. 14. P. 778–787. https://doi.org/10.1007/s10495-009-0349-3
- Isakova A.A., Artykov A.A., Plotnikova E.A., Trunova G.V., Khokhlova V.А., Pankratov A.A., Shuvalova M.L., Mazur D.V., Antipova N.V., Shakhparonov M.I., Dolgikh D.A., Kirpichnikov M.P., Gasparian M.E., Yagolovich A.V. // Int. J. Biol. Macromol. 2024. V. 255. P. 128096. https://doi.org/10.1016/j.ijbiomac.2023.128096
- Bartha Á., Győrffy B. // Int. J. Mol. Sci. 2021. V. 22. P. 2622. https://doi.org/10.3390/ijms22052622
- Beaufort C.M., Helmijr J.C., Piskorz A.M., Hoogstraat M., Ruigrok-Ritstier K., Besselink N., Murtaza M., Van IJcken W.F., Heine A.A., Smid M., Koudijs M.J., Brenton J.D., Berns E.M., Helleman J. // PLoS One. 2014. V. 9. P. e103988. https://doi.org/10.1371/journal.pone.0103988
- DelloRusso C., Welcsh P.L., Wang W., Garcia R.L., King M.-C., Swisher E.M. // Mol. Cancer Res. 2007. V. 5. P. 35–45. https://doi.org/10.1158/1541-7786.MCR-06-0234
- Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., Van Den Ouweland A., Merajver S.D., Ethier S.P., Schutte M. // Cancer Res. 2006. V. 66. P. 41–45. https://doi.org/10.1158/0008-5472.CAN-05-2853
- Gasparian M.E., Bychkov M.L., Yagolovich A.V., Dolgikh D.A., Kirpichnikov M.P. // Biochem. (Moscow). 2015. V. 80. P. 1080–1091. https://doi.org/10.1134/S0006297915080143
- Chou T.-C. // Cancer Res. 2010. V. 70. P. 440– 446. https://doi.org/10.1158/0008-5472.CAN-09-1947
Қосымша файлдар
