Interaction of Lyophilic Zinc(II) Porphyrins with Bovine Serum Albumin

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Palladium-catalyzed heterylation of monobromophenyl-substituted zinc(II) porphyrin with small heterocycles (benzothiazole, benzoxazole, and N-methylbenzimidazole) was carried out. As a result, unsymmetrical heterylphenyl-substituted zinc(II) porphyrins soluble in organic solvents were obtained. The interaction of heteryl-substituted zinc(II) porphyrins with alpha-helical proteins was studied by spectral methods using bovine serum albumin in aqueous organic solvents. It was found that the titration of the zinc(II) porphyrins with albumin in a sodium phosphate buffer involves a number of equilibria including complexation and aggregation. In the case of porphyrins containing N-methylbenzimidazole and benzoxazole residues, self-aggregation processes initiated by absorption of organic solvent molecules by the protein predominate. It was found that more hydrophobic nature of zinc(II) porphyrin with benzothiazole residue promotes the complex formation with the protein. The photochemical properties of zinc(II) porphyrin with a benzothiazole residue, capacity for the photooxidation of the alpha-helical protein, and the high affinity of protein to this porphyrin make it a promising candidate for the potential applicability for photodynamic inactivation.

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作者简介

O. Koifman

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

N. Lebedeva

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

E. Yurina

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

Yu. Gubarev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

S. Syrbu

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

A. Kiselev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

M. Lebedev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

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2. Scheme 1. Synthesis of asymmetric heteryl-substituted zinc(II)porphyrins

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3. Fig. 1. ZnPorO ESP (8.210-6 M) (a) and its fluorescence spectrum (b) in DMFA (dotted line) and PBS-DMFA (0.19 M) (solid line).

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4. Fig. 2. ESP ZnPorS (4.9910-6 M) (a) and ZnPorN (6���10-6 M) (b) in PBS-DMFA –0.19 M) during titration of BSA (0-6���10-6 M.

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5. Fig. 3. Corrected fluorescence spectra of BSA (2.2410-5 M) during ZnPorS titration (0-7.82���10-6 M) in PBS–DMFA (0.19 M), taking into account the absorption of ZnPorS.

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6. Fig. 4. Constants of the observed rate of photooxidation of BSA in the presence of ZnPorX under irradiation with 425 nm light, estimated as the ratio of BSA fluorescence before irradiation (lnF0) to BSA fluorescence (Fi) under irradiation.

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