ASSOCIATION OF MICRORNA EXPRESSION WITH EARLY STAGE OF BREAST CANCER METASTASIS

Abstract

A serious complication of breast cancer is metastasis of tumor cells. The initiation of this process takes place in the nearby lymphatic vessels. To date, the molecular mechanisms of metastasis are not well understood and further research is required. In this regard, the level of miRNA expression can provide information both on the mechanisms of metastasis development and also be a source of markers for the prognosis of metastatic breast cancer. In the present work, according to the literature, we selected four microRNAs (miR-34a, miR-145, miR-125b and miR-222), potentially capable of being associated with metastasis, to study their association with metastasis to lymph nodes at an early stage. The measurement of the expression of four selected miRNAs on 40 paired samples (tumor-norm) was performed using real-time PCR. Frequencies of miR-34a, miR-145, miR-125b, and miR-222 relative to normal tissue were determined for reduced or increased miR-mRNA expression. For a more detailed study of the association of miRNA expression with lymph node metastasis, miR-125b and miR-222 were selected. An association of expression of these two miRNAs was found with the onset of metastasis to the lymph nodes: with the same level of confidence, differences (p = 0.01). Both miRNAs (miR-125b and miR-222) are involved in the metastasis of breast cancer, apparently by influencing the epithelial-mesenchymal transition. They can potentially act as prognostic biomarkers to assess the likelihood of early lymph node metastases in breast cancer.

Full Text

Restricted Access

About the authors

Kristina A. Grishina

Research Centre for Medical Genetics (RCMG)

Email: grstina@yandex.ru
researcher of the Laboratory of molecular genetics of complex inherited diseases RCMG 115522, Moscow, Russian Federation

N. I Pospekhova

Research Centre for Medical Genetics (RCMG)

115522, Moscow, Russian Federation

V. A Khaylenko

N.N. Blokhin National Medical Research Center of Oncology оf the Ministry of Health of the Russian Federation

115478, Moscow, Russian Federation

A. V Karpukhin

Research Centre for Medical Genetics (RCMG)

115522, Moscow, Russian Federation

References

  1. https://www.who.int/cancer/prevention/diagnosis-screening/breast-cancer/en/
  2. Woods R. W., Camp M., Durr N. J., Harvey S. (Accepted/In press). A Review of Options for Localization of Axillary Lymph Nodes in the Treatment of Invasive Breast Cancer. AcademicRadiology. https://doi.org/10.1016/j.acra.2018.07.002
  3. Гришина К.А., Хайленко В.А., Хайленко Д.В., Карпухин А.В. Роль микроРНК в развитии РМЖ и их потенциал в качестве биомаркеров этого заболевания. Опухоли женской репродуктивной системы. 2018; 15 (3): 40-7. https://doi.org/10.17650/1994-4098-2018-14-3-40-47
  4. Hu J., Guo H., Li H., Liu Y., Liu J. et al. Retraction: MiR-145 Regulates Epithelial to Mesenchymal Transition of Breast Cancer Cells by Targeting Oct4. PLoS One. 2013; 8 (5): 10.1371/annotation/aaef10de-ee36-416f-93eb-959dfa3dc92e. https://doi.org/10.1371/annotation/aaef10de-ee36-416f-93eb-959dfa3dc92e
  5. Feng Tang, Rui Zhang, Yunmian He, Meijuan Zou, Le Guo, Tao Xi. MicroRNA-125b Induces Metastasis by Targeting STARD13 in MCF-7 and MDA-MB-231 Breast Cancer Cells. PLoS One. 2012; 7 (5): e35435. https://doi.org/10.1371/journal.pone.0035435
  6. Chernyy V., Pustylnyak V., Kozlov V., Gulyaeva L. Increased expression of miR-155 and miR-222 is associated with lymph node positive status. Journal of Cancer. 2018; 9 (1): 135-40. doi: 10.7150/jca.22181.
  7. Xiaoping Rui, Haibin Zhao, Xianqiu Xiao, Li Wang, Lin Mo, Yao Yao. MicroRNA-34a suppresses breast cancer cell proliferation and invasion by targeting Notch1. Exp.Ther. Med. 2018; 16 (6): 4387-92. doi: 10.3892/etm.2018.6744
  8. Paola Bonetti, Montserrat Climent, Fabiana Panebianco, Chiara Tordonato, Angela Santoro, Matteo Jacopo Marzi, Pier Giuseppe Pelicci, Andrea Ventura & Francesco Nicassio. Dual role for miR-34a in the control of early progenitor proliferation and commitment in the mammary gland and in breast cancer. Oncogene. 2019; 38: 360-74. https://doi.org/10.1038/s41388-018-0445-3
  9. Yilmaz M., Christofori G. EMT, the cytoskeleton, and cancer cell invasion. Cancer Metastasis Rev. 2009; 28: 15. https://doi.org/10.1007/s10555-008-9169-0
  10. Yanwei Luo Xinye Wang Weihong Niu Heran Wang Qiuyuan Wen Songqing Fan Ran Zhao Zheng Li Wei XiongShuping Peng Zhaoyang Zeng Xiaoling Li Guiyuan Li Ming Tan Ming Zhou. Elevated microRNA-125b levels predict a worse prognosis in HER2-positive breast cancer patients. Oncology letters. 2016: 867-74. https://doi.org/10.3892/ol.2016.5482

Statistics

Views

Abstract: 133

Article Metrics

Metrics Loading ...

Refbacks

  • There are currently no refbacks.

Copyright (c) 2018 Eco-Vector



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies