Exosomal microRNAs in colorectal cancer diagnostics



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BACKGROUND: Global statistics demonstrate an upward trend in colorectal cancer (CRC) incidence and mortality, especially in developed and developing countries, in young working-age individuals under 50 years of age, which makes the search for non-invasive CRC markers relevant.

AIM: To identify target exosomal microRNAs for CRC diagnostics in patients over and under 50 years of age.

MATERIALS AND METHODS: Next-generation sequencing (NGS) of blood plasma samples from 10 patients with CRC was performed, followed by an assessment of the hydrodynamic size of exosomes using dynamic light scattering in three samples.

RESULTS: Nine molecules of exosomal microRNAs most characteristic of CRC patients were identified. For patients over 50 years old: miR-26 b, miR-124-1, miR-518 b, miR-520 d, miR-518 d, miR-612, miR-1283-1, miR-1260 a, for patients under 50 years old: miR-4510.

CONCLUSIONS. Non-invasive diagnostics of colorectal cancer using exosomal microRNAs has a high diagnostic potential in individuals of different age groups, but given the difficult-to-reproduce methodology in routine clinical practice, it requires further scientific research.

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作者简介

Margarita Serbaeva

Krasnoyarsk Regional Clinical Oncology Dispensary named after A.I. Kryzhanovsky, Krasnoyarsk, Russia; Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia

Email: serbaeva94@mail.ru
ORCID iD: 0009-0007-3268-2535
SPIN 代码: 4662-4362

Radiotherapist at the Radiotherapy Department No. 2, Assistant at the Department of Oncology and Radiation Therapy with a course PO

俄罗斯联邦, Krasnoyarsk, Russia; Krasnoyarsk, Russia

Sergei Vorobyev

Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science, Krasnoyarsk, Russia

Email: yekspatz@yandex.ru
SPIN 代码: 7747-0299

MD, Cand. Sci. (Medicine)

俄罗斯联邦, Krasnoyarsk, Russia

Tatyana Ruksha

Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia

Email: tatyana_ruksha@mail.ru
ORCID iD: 0000-0001-8142-4283
SPIN 代码: 5412-2148

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Krasnoyarsk, Russia

Ruslan Zukov

Krasnoyarsk Regional Clinical Oncology Dispensary named after A.I. Kryzhanovsky, Krasnoyarsk, Russia; Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia

编辑信件的主要联系方式.
Email: zukov.ra@krasgmu.ru
ORCID iD: 0000-0002-7210-3020
SPIN 代码: 3632-8415
Researcher ID: A-8193-2016

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Krasnoyarsk, Russia; Krasnoyarsk, Russia

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
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