Exosomal microRNAs in Diagnosis of Colorectal Cancer
- Authors: Serbaeva M.S.1,2, Vorobyev S.A.3, Ruksha T.G.2, Zukov R.A.1,2
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Affiliations:
- Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary
- Professor Voino-Yasenetsky Krasnoyarsk State Medical University
- Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Sciences
- Issue: Vol 30, No 1 (2025)
- Pages: 17-24
- Section: Original Study Articles
- Submitted: 17.12.2024
- Accepted: 14.05.2025
- Published: 23.06.2025
- URL: https://rjonco.com/1028-9984/article/view/643119
- DOI: https://doi.org/10.17816/onco643119
- EDN: https://elibrary.ru/WJALWU
- ID: 643119
Cite item
Abstract
BACKGROUND: Global statistics show that the incidence and mortality rates of colorectal cancer are rising, particularly among young adults (< 50 years of age) in developed and developing countries. This requires the search for non-invasive colorectal cancer biomarkers.
AIM: The study aimed to identify exosomal microRNA targets for diagnosing colorectal cancer in patients over and under 50 years of age.
METHODS: Next-generation sequencing was performed on plasma samples from 10 patients (6 men and 4 women) between the ages of 32 and 65 diagnosed with colorectal cancer followed by hydrodynamic diameter analysis of exosomes measured by dynamic light scattering in triplicate.
RESULTS: Nine microRNAs that are characteristic of colorectal cancer were identified in exosomes: miR-26b, miR-124-1, miR-518b, miR-520d, miR-518d, miR-612, miR-1283-1, and miR-1260 for patients > 50 years, and miR-4510 for patients < 50 years.
CONCLUSION: The non-invasive diagnosis of colorectal cancer using exosomal microRNAs shows high potential across age groups. However, challenges in reproducing this technique in routine clinical practice require further research.
Full Text

About the authors
Margarita S. Serbaeva
Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary; Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Author for correspondence.
Email: serbaeva94@mail.ru
ORCID iD: 0009-0007-3268-2535
SPIN-code: 4662-4362
Russian Federation, Krasnoyarsk; Krasnoyarsk
Sergey A. Vorobyev
Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Sciences
Email: yekspatz@yandex.ru
SPIN-code: 7747-0299
MD, Cand. Sci. (Chemistry)
Russian Federation, KrasnoyarskTatiana G. Ruksha
Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Email: tatyana_ruksha@mail.ru
ORCID iD: 0000-0001-8142-4283
SPIN-code: 5412-2148
MD, Dr. Sci. (Medicine), Professor
Russian Federation, KrasnoyarskRuslan A. Zukov
Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary; Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Email: zukov_rus@mail.ru
ORCID iD: 0000-0002-7210-3020
SPIN-code: 3632-8415
MD, Dr. Sci. (Medicine), Professor
Russian Federation, Krasnoyarsk; KrasnoyarskReferences
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