Email this article Exosomal microRNAs in Diagnosis of Colorectal Cancer
- Authors: Serbaeva M.S.1,2, Vorobyev S.A.3, Ruksha T.G.2, Zukov R.A.1,2
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Affiliations:
- Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary
- Professor Voino-Yasenetsky Krasnoyarsk State Medical University
- Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Sciences
- Issue: Vol 30, No 1 (2025)
- Pages: 17-24
- Section: Original Study Articles
- Submitted: 17.12.2024
- Accepted: 14.05.2025
- Published: 23.06.2025
- URL: https://rjonco.com/1028-9984/article/view/643119
- DOI: https://doi.org/10.17816/onco643119
- EDN: https://elibrary.ru/WJALWU
- ID: 643119
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Open Access
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Abstract
BACKGROUND: Global statistics show that the incidence and mortality rates of colorectal cancer are rising, particularly among young adults (< 50 years of age) in developed and developing countries. This requires the search for non-invasive colorectal cancer biomarkers.
AIM: The study aimed to identify exosomal microRNA targets for diagnosing colorectal cancer in patients over and under 50 years of age.
METHODS: Next-generation sequencing was performed on plasma samples from 10 patients (6 men and 4 women) between the ages of 32 and 65 diagnosed with colorectal cancer followed by hydrodynamic diameter analysis of exosomes measured by dynamic light scattering in triplicate.
RESULTS: Nine microRNAs that are characteristic of colorectal cancer were identified in exosomes: miR-26b, miR-124-1, miR-518b, miR-520d, miR-518d, miR-612, miR-1283-1, and miR-1260 for patients > 50 years, and miR-4510 for patients < 50 years.
CONCLUSION: The non-invasive diagnosis of colorectal cancer using exosomal microRNAs shows high potential across age groups. However, challenges in reproducing this technique in routine clinical practice require further research.
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About the authors
Margarita S. Serbaeva
Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary; Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Author for correspondence.
Email: serbaeva94@mail.ru
ORCID iD: 0009-0007-3268-2535
SPIN-code: 4662-4362
Russian Federation, Krasnoyarsk; Krasnoyarsk
Sergey A. Vorobyev
Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Sciences
Email: yekspatz@yandex.ru
SPIN-code: 7747-0299
MD, Cand. Sci. (Chemistry)
Russian Federation, KrasnoyarskTatiana G. Ruksha
Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Email: tatyana_ruksha@mail.ru
ORCID iD: 0000-0001-8142-4283
SPIN-code: 5412-2148
MD, Dr. Sci. (Medicine), Professor
Russian Federation, KrasnoyarskRuslan A. Zukov
Kryzhanovsky Krasnoyarsk Regional Clinical Oncology Dispensary; Professor Voino-Yasenetsky Krasnoyarsk State Medical University
Email: zukov_rus@mail.ru
ORCID iD: 0000-0002-7210-3020
SPIN-code: 3632-8415
MD, Dr. Sci. (Medicine), Professor
Russian Federation, Krasnoyarsk; KrasnoyarskReferences
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