High expression of CD8+ T-lymphocytes in the peritumoral zone of renal cancer as a factor of unfavorable prognosis

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Abstract

AIM: To evaluate the prognostic value of quantitative CD8+ T-lymphocyte counts in the peritumorotic zone of renal cell cancer.

MATERIAL AND METHODS: The material of 74 patients after operative treatment for renal cell carcinoma with complete 5-year follow-up was retrospectively analyzed. Of the 74 patients, 56 were alive by the 5-year follow-up, and 18 had died of cancer. After immunohistochemical staining, CD8+ T-lymphocyte distribution density in the peritumorotic zone of carcinomas was determined in 3 representative fields of view at ×400 magnification and correlated with the clinicopathological characteristics of neoplasms and overall 5-year patient survival rate.

RESULTS: The high level of CD8+ T-lymphocyte infiltration in the peritumoral zone of carcinomas was seen in stages III–IV of the disease (p=0.0000001), in tumors >7 cm (p=0.0000001), in Fuhrman grade III–IV anaplasia (p=0.001), in metastases (p=0.0000001) and in the shorter total survival rate of patients (p=0.0003). High CD8+ T-lymphocyte count was also observed in men (p=0.001) and in cases of unclassified cancer.

CONCLUSION: In the light of the need to search for new prognostic criteria in renal cancer, we conclude that a high level of peritumorous zone infiltration by CD8+ T-lymphocytes is an unfavorable prognostic factor. Assessment of peritumorous zone infiltration by CD8+ T-lymphocytes may serve as an additional prognostic factor in renal cell carcinoma.

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About the authors

Igor P. Bobrov

Altai State Medical University

Author for correspondence.
Email: ig.bobrov2010@yandex.ru
ORCID iD: 0000-0001-9097-6733
SPIN-code: 2375-1427

MD, Dr. Sci (Med.), Professor

Russian Federation, Barnaul

Alexander F. Lazarev

Altai State Medical University

Email: lazarev@arzs.ru
ORCID iD: 0000-0003-1080-5294
SPIN-code: 1161-8387

MD, Dr. Sci. (Med.), Professor

Russian Federation, Barnaul

Tatyana M. Cherdantseva

Ryazan State Medical University named after Academician I.P. Pavlov

Email: cherdan.morf@yandex.ru
ORCID iD: 0000-0002-7292-4996
SPIN-code: 3773-8785

MD, Dr. Sci. (Med.), Associate Professor

Russian Federation, Ryazan

Ilya V. Klimachev

Altai State Medical University

Email: k0222@yandex.ru
ORCID iD: 0000-0001-9889-9517

MD

Russian Federation, Barnaul

Vladimir V. Klimachev

Altai State Medical University

Email: pathology_agmu@mail.ru
ORCID iD: 0000-0003-1586-252X
SPIN-code: 8120-3059

MD, Dr. Sci. (Med.), Professor

Russian Federation, Barnaul

Mikhail N. Myadelets

Altai State Medical University

Email: pathology_agmu@mail.ru
ORCID iD: 0000-0003-3256-6706

MD, Cand. Sci. (Med.)

Russian Federation, Barnaul

Alexander V. Lepilov

Altai State Medical University

Email: lepilov@list.ru
ORCID iD: 0000-0003-2477-4687
SPIN-code: 7516-0963

MD, Dr. Sci. (Med.), Professor

Russian Federation, Barnaul

Andrey Yu. Dolgatov

Altai State Medical University

Email: adolgatov@yandex.ru
ORCID iD: 0000-0002-7677-4622
SPIN-code: 2804-0011

MD, Cand. Sci. (Med.), Associate Professor

Russian Federation, Barnaul

Nikolay A. Korsikov

Altai State Medical University

Email: nikkor94knaagmu@yandex.ru
ORCID iD: 0000-0002-0094-4656
Russian Federation, Barnaul

Elena S. Dolgatova

Altai State Medical University

Email: esdol82@yandex.ru
ORCID iD: 0000-0002-6678-4472
Russian Federation, Barnaul

Elena L. Lushnikova

Institute of Molecular Pathology and Pathomorphology of the Federal Research Center for Fundamental and Translational Medicine

Email: pathol@inbox.ru
ORCID iD: 0000-0002-2614-8690
SPIN-code: 3310-1175

MD, Dr. Sci. (Med.), Professor

Russian Federation, Novosibirsk

Maxim A. Bakarev

Institute of Molecular Pathology and Pathomorphology of the Federal Research Center for Fundamental and Translational Medicine

Email: pathol@inbox.ru
ORCID iD: 0000-0002-9614-592X
SPIN-code: 3630-9977

MD, Dr. Sci. (Med.), Professor

Russian Federation, Novosibirsk

References

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2. Fig. 1. Distribution density of CD8+ T-lymphocytes in peritumoral zone depending on the degree of anaplasia according to Fuhrman: a — small number of CD8+ T-lymphocytes, increase ×400; b — significant number of CD8+ T-lymphocytes at degree IV, increase ×400. Immunohistochemical staining for CD8.

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3. Fig. 2. Survival curves as a function of CD8+ T-lymphocyte distribution density in peritumoral zone.

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